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KMID : 0880220230610010083
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Journal of Microbiology
2023 Volume.61 No. 1 p.83 ~ p.93
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Genome Sequencing Highlights the Plant Cell Wall Degrading Capacity of Edible Mushroom Stropharia rugosoannulata
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Mengpei Guo
Xiaolong Ma
Yan Zhou
Yinbing Bian
Gaolei Liu
Yingli Cai
Tianji Huang
Hongxia Dong
Dingjun Cai
Xueji Wan
Zhihong Wang
Yang Xiao
Heng Kang
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Abstract
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The basidiomycetous edible mushroom Stropharia rugosoannulata has excellent nutrition, medicine, bioremediation, and biocontrol properties. S. rugosoannulata has been widely and easily cultivated using agricultural by-products showing strong lignocellulose degradation capacity. However, the unavailable high-quality genome information has hindered the research on gene function and molecular breeding of S. rugosoannulata. This study provided a high-quality genome assembly and annotation from S. rugosoannulata monokaryotic strain QGU27 based on combined Illumina-Nanopore data. The genome size was about 47.97 Mb and consisted of 20 scaffolds, with an N50 of 3.73 Mb and a GC content of 47.9%. The repetitive sequences accounted for 17.41% of the genome, mostly long terminal repeats (LTRs). A total of 15,726 coding gene sequences were putatively identified with the BUSCO score of 98.7%. There are 142 genes encoding plant cell wall degrading enzymes (PCWDEs) in the genome, and 52, 39, 30, 11, 8, and 2 genes related to lignin, cellulose, hemicellulose, pectin, chitin, and cutin degradation, respectively. Comparative genomic analysis revealed that S. rugosoannulata is superior in utilizing aldehyde-containing lignins and is possible to utilize algae during the cultivation.
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KEYWORD
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Stropharia rugosoannulata, Whole-genome sequencing, Plant cell wall degrading enzymes, Comparative genomic analysis
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